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KMID : 1123920100240020220
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Korean Journal of Oriental Physiology and Pathology
2010 Volume.24 No. 2 p.220 ~ p.227
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( Effects of Puerariae Radix extract on Cisplatin-Induced Apoptosis of Rat Mesangial Cells )
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Hong Jae-Eui
Shin Jo-Young
Ju Sung-Min
Jeon Byung-Hoon
Lee Si-Hyeong
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Abstract
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Puerariae radix (PR) is a popular natural herb and a traditional food in Asia, which has antithrombotic and anti-allergic properties and stimulates estrogenic activity. One of the major side effects of cisplatin is nephrotoxicity, leading to acute renal failure. Recent study has suggested a role of ROS and p53 in renal cell injury by cisplatin. We studied that protective effects of PR on cisplatin-induced apoptosis in rat mesangial cell. Rat mesangial cell was preincubated with PR (50, 100, 150 and 200 ) for 12 hr and then treated with 30 cisplatin for 24 hr. Protective effect of PR on cisplatin-induced apoptosis in ECV304 cells was determined using MTT assay, FDA-PI staining, flow cytometric analysis, caspase-3 activity assay, ROS assay and western blot. Our results showed that PR inhibited in cisplatin-induced apoptosis and ROS production in ECV304 cells. Moreover, PR reduced ERK, p38 and JNK activation that increased in cisplatin-treated rat mesangial cell. Furthermore, activation of p53 by cisplatin in rat mesangial cell was inhibited by PR treatment. These results suggest that protective effect of PR on cisplatin-induced apoptosis in rat mesangial cell may be associated with reduction of ERK, p38, JNK, p53 activation.
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KEYWORD
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Puerariae Radix, ROS, ERK, p38, JNK
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